Urine assay detects oral semaglutide metabolites for 28 hours, researchers report

The wire

1. 01A UHPLC-HRMS method reached a 0.03 ng/mL limit of detection for semaglutide metabolites U6 and U7; after a single 3 mg oral microdose they appeared at 21 hours and persisted to 28 hours.
2. 02Authors frame semaglutide misuse as a risk in weight-class sports, and the method gives testing labs a validated route to screen for the oral formulation.
3. 03The excretion profile comes from one healthy volunteer across 25 post-dose samples, so detection windows at therapeutic doses and across populations remain unestablished.

From the source

Detection and Excretion Profiling of Semaglutide Use in Human Urine by UHPLC-HRMS for Anti-Doping Purpose

Drug testing and analysis · 2026 Oct 2 · Tian T, Chen X, Deng X et al.